Next-generation DNA-RNA Sequencing


Next Generation DNA · RNA sequencing: "Quick delivery", "Reasonable", and "Customized"

It is possible to narrow down the genes, genomic regions, and microorganisms that are important in creating specific constitutions, cells, environmental conditions, and to predict their function even in situations without prior information / knowledge, by genomic, epi-genome and meta-genomic analysis at the whole genome level using the next generation sequencer. Our DNA / RNA sequencing service supports research and development using the next generation sequencer in various situations based on the three concepts of "short delivery time", "reasonable" and "customization".

Plan Time for delivery A price/run The number of the maximum leads Lead length Data volume The main uses
PE-LR-1001 Five business days *1 200,000 yen *2, 3 8 millions 150bp pair-end 2.1~2.4Gb 16S rRNA system analysis
(4~48 samples)
SE-SR-1001 Five business days 250,000 yen 25 millions 75bp single-end 1.65~1.875Gb ChIP-seq QC *4
MBD-seq QC
PE-SR-1001 Five business days 300,000 yen 25 millions 75bp pair-end 3.3~3.75Gb RNA-seq QC
Targeted/Small RNA-seq
PE-LR-1002 Five business days 400,000 yen 25 millions 150bp pair-end 6.6~7.5Gb 16S rRNA phylogenetic analysis (48-96 samples)
Target resequencing

*1 Regarding the sequencing order within 5 business days, please tell us when you contact us. *2 In the case of sequencing within 5 business days, you may be asked to the additional cost 10 to 30% of the set price. * 3 This is the set price when sequencing is performed for library adjusted sample (Illumina sequencing adapter is added). If the library is not adjusted, we will charge an additional processing fee of 20,000 yen per sample. *4 QC: The abbreviation for Quality Check

We do a genome-wide quality check before deep sequencing of ChIP-seq, RNA-seq, and other genome / epi-genome data. It is possible to select very accurate samples and estimate the appropriate number of deep sequencing leads, by genome-wide quality check.

(The expense of a genome wide and quality check is included in the default price of each plan.)

Quality check of lead enrichment, and generation and evaluation of virtual deep sequencing data by lead number correction

Total number of leads, lead duplication ratio derived from PCR duplicates, peak area number, lead concentration ratio in peak area are evaluated comprehensively.

Tumor profiling-target resequencing

It is possible to predict driver mutations / polymorphisms of cancer and other diseases efficiently and inexpensively, by focusing on specific genomic and genomic regions. You can use broadly not only at research and development but at the clinical site.

Plan Time for delivery A price/sample Analysis outline
Tumor profiling Ten business days 80,000 yen
(from 8 samples)
DNA sequencing targeted to cancer-related 15 genes (AKT1, GNA11, NRAS, BRAF , GNAQ, PDGFRA, EGFR, KIT, PIK3CA, ERBB2, KRAS, RET, FOXL2, MET, TP53).
Target resequencing Ten business days 150,000 yen
(from 3 samples)
DNA sequencing targeted to disease-related 4,813 genes. DNA sequencing of the user-specified region by the custom of the target sequencing panel.

Expression analysis of target gene, and small RNA

By focusing on specific genes, it is possible to predict gene expression patterns related to cancer and disease mainly efficiently and cheaply. In addition, we will comprehensively analyze the expression patterns of microRNAs that are drawing attention as new molecular markers of diseases and narrow them down.

Plan Time for delivery A price/sample Analysis outline
Targeted RNA-seq Ten business days 60,000 yen
(from 8 samples)
Revelation quantity analysis of the specific gene using the exclusive panel. It covers 50 to 100 genes to be involved in Apoptosis, NFkB pathway, P450 pathway, P53 pathway, stem cell, Wnt pathway, cardiotoxicity, cell cycle, hedgehog pathway, and neurodegeneration.
Small RNA-seq Ten business days 100,000 yen
(from 4 samples)
Comprehensive expression quantitative analysis of small RNA (17 to 34 bp single-stranded noncoding RNA) such as microRNA (miRNA). We search dedicated database and integrate annotation / variant information.

Microbe DNA complete coverage analysis in a living body, environment, and food

Microbe DNA complete coverage analysis of "Quick delivery", "Streamlined production system", and "Original analysis platform"

By microbial DNA coverage analysis (following "16S rRNA phylogenetic analysis"), it is possible to narrow down the microorganisms involved in maintaining and changing specific conditions. We will receive your order in bulk, such as DNA extraction from soil, water, intestinal tract, food sample, production and sequencing of 16S rRNA amplicon, clustering of microbial flora, and narrowing down target microorganisms. Furthermore, it is possible to identify new associations with macro phenomena, by integrating the microflora distribution and metadata with our unique analysis platform.


Sample type *1 Time for delivery A price/sample
(4 samples) ※2
A price/sample
(5~12 samples)
A price/sample
(13~24 samples)
A price/sample
(25~48 samples)
A price/sample
(49~96 samples)
Soil Ten business days 140,000 yen 100,000 yen 80,000 yen 60,000 yen 40,000 yen
Water and solution Ten business days 140,000 yen 100,000 yen 80,000 yen 60,000 yen 40,000 yen
Food Ten business days 140,000 yen 100,000 yen 80,000 yen 60,000 yen 40,000 yen
Feces Ten business days 160,000 yen 120,000 yen 100,000 yen 80,000 yen 60,000 yen

The required amount depends on the sample, please contact us for details. ※2 If samples are less than 4, we might decline your order.

The outline of 16S rRNA system analysis

We will comprehensively analyze the sequence pattern diversity of the coding region of 16S rRNA (small subunit of ribosome) common to microorganisms and clarify the microflora distribution in the sample.

    

Narrow down target microorganisms by original algorithm and integrated analysis using machine learning

Following the phylogenetic analysis using the dedicated analysis program QIIME, we do clustering analysis using unique calculation algorithm and narrow down class-specific microorganisms. Furthermore, We will clarify the relationship between the change of microflora and more macro phenomena, by dedicated analysis based on Deep Learning method, the result of 16S rRNA phylogenetic analysis and other metadata (sampling condition, treatment method, other measurement data) are integratedly analyzed.

Access

  • 〒101-0032
  • 3-7-4 3F Iwamotocho, Chiyoda-ku, Tokyo, Japan

Contact

  • E-mail:info@rhelixa.co.jp
  • TEL:+81-3-6240-9330
  • FAX:+81-3-6240-9331