NGS phylogenetic analysis using target amplicon

Microbial floral analysis from environment, food and feces

Using microbial DNA coverage analysis (following “16S rRNA phylogenetic analysis”), we can provide the layout that characterizes the phylogeny of bacteria. This is done by extracting DNA from samples (soil, water, gut, food samples,etc.) and sequencing the species-specific 16S rRNA region. Furthermore, we can provide insights to the functionality of the organisms from the metadata we obtained though our own analysis platform.

The required amount and delivery method will differ among the types of samples. Please contact us for details.

PlanSequencerCost/Sample
(1〜4 Samples)
Cost/Sample
(5~12 Samples)
Cost/Sample
(13~24 Samples)
Cost/Sample
(>25 Samples)
Metagenome Experiment and AnalysisMiniSeq (V4 Amplicon)
MiSeq (V3-4Amplicon)
120 000 yen100 000 yen80 000 yen60 000 yen
Experiment ONLY
(until raw data in fast. format)
MiniSeq (V4Amplicon)
MiSeq (V3-4Amplicon)
100 000 yen80 000 yen60 000 yen40 000 yen
Analysis ONLYMiniSeq (V4Amplicon)
MiSeq (V3-4Amplicon)
100 000 yen80 000 yen60 000 yen40 000 yen

*For MiSeq analysis (V3-4 amplicon), there will be an additional charge of 10000 yen for every sample requested.。

About 16S rRNA phylogenetic analysis

We analyze the sequence pattern diversity of the coding region of 16S rRNA (small subunit of ribosome) common to all microorganisms and clarify the microflora distribution in the sample.

* Phylogenetic analysis targeting the V1 – V3 and V3 – V4 areas is also available

Machine learning-based integrated analysis

Following the phylogenetic analysis using the QIIME program, the diversity of the microorganisms are further classified using our own algorithm. Furthermore, utilizing Deep-Learning method, we perform integrated analysis from the result of 16S rRNA phylogenetic analysis and other metadata (sampling condition, treatment method, other measurement data) to help elucidate the relationship between the microorganism and its surrounding environment.

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